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stet buffer  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd stet buffer
    Stet Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 879 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stet+buffer/EDTA/pm42019502-152-38-57
    Average 96 stars, based on 879 article reviews
    stet buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Extraction:

    Article Title: Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variation.
    Article Snippet: .. Cells were harvested at OD600[0.3–0.5] and pelleted at 3,220 xg for 12 min. Pellets were resuspended in 10 μL dPBS for HMW gDNA extraction using the Nanobind CBB kit (PacBio 102-301-900), including an extra lysis in 50 μL STET buffer [50 mM Tris-HCl, 50 mM EDTA, 8% (w/v) sucrose, 5% (w/v) Triton X-100, 10 mg.mL− 1 lysozyme (MP Biomedicals 100831), and 0.2 mg.mL− 1 lysostaphin (Sigma L7386)] as recommended by the manufacturer. ..

    Lysis:

    Article Title: Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variation.
    Article Snippet: .. Cells were harvested at OD600[0.3–0.5] and pelleted at 3,220 xg for 12 min. Pellets were resuspended in 10 μL dPBS for HMW gDNA extraction using the Nanobind CBB kit (PacBio 102-301-900), including an extra lysis in 50 μL STET buffer [50 mM Tris-HCl, 50 mM EDTA, 8% (w/v) sucrose, 5% (w/v) Triton X-100, 10 mg.mL− 1 lysozyme (MP Biomedicals 100831), and 0.2 mg.mL− 1 lysostaphin (Sigma L7386)] as recommended by the manufacturer. ..



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